Ph.D. Sabine Schneider
Tel: +49 (0)89 289-12891
Dynamics of the interactions between conformational restricted cyclic peptides and structured RNA elements
Cyclic peptides (ZP) belong to the most effective, high-affinity drug-like agents, which are extremely stable, flexible, variable and exhibit a wide range of biological activities. Aim of the here proposed project is to establish an in vivo screening assay to identify ZPs from an expressed library, specifically binding to structured and conserved RNA elements in bacterial mRNAs in order to inhibit growth of pathogenic bacteria. These ZP-RNA interactions will be biochemical, biophysical and structural characterised and their conformational states theoretically and biophysically analysed. Based on these results, chemical modifications influencing the dynamics of the ZPs will be introduced, resulting in an increased binding probability and affinity to the target RNA.